論文題目是：不同酒精濃度和不同酒精倍數(shù)對(duì)灰樹花265多糖提取的影響

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目錄
摘要 ………………………………………………………………………………………………3
Abstract  …………………………………………………………………………………………4
1引言 ……………………………………………………………………………………………5
2材料與方法 ……………………………………………………………………………………7
2.1 材料…………………………………………………………………………………………7
2.2試劑 …………………………………………………………………………………………7
2.3儀器設(shè)備……………………………………………………………………………………7
2.4 發(fā)酵液的處理 ……………………………………………………………………………8
2.5 酒精提取……………………………………………………………………………………8
2.6 酮-比色法 …………………………………………………………………………………8
2.6.1 原理………………………………………………………………………………………8
2.6.2 藥品………………………………………………………………………………………8
2.6.3 試劑………………………………………………………………………………………8
2.6.4葡萄糖標(biāo)準(zhǔn)液……………………………………………………………………………8
2.6.5樣品總糖的測(cè)定…………………………………………………………………………9
2.6.6樣品還原糖的測(cè)定 ……………………………………………………………………9
2.6.7計(jì)算 ………………………………………………………………………………………9
３結(jié)果與分析 …………………………………………………………………………………10
3.1.1不同酒精倍數(shù)的醇沉樣品中總糖含量測(cè)定 ……………………………………10
3.1.2不同酒精濃度的醇沉樣品中總糖含量測(cè)定 ……………………………………11
3.2.1不同酒精倍數(shù)的醇沉樣品中還原糖含量測(cè)定 …………………………………11 
3.2.2不同酒精濃度的醇沉樣品中還原糖含量測(cè)定 …………………………………12
3.3.1不同酒精倍數(shù)的醇溶樣品中總糖含量測(cè)定 ……………………………………13
3.3.2不同酒精濃度的醇溶樣品中總糖含量測(cè)定 ……………………………………14
3.4.1不同酒精倍數(shù)的醇溶樣品中還原糖含量測(cè)定 …………………………………14
3.4.2不同酒精濃度的醇溶樣品中還原糖含量測(cè)定 …………………………………15
4分析與討論……………………………………………………………………………………16
致謝………………………………………………………………………………………………16
參考文獻(xiàn) ………………………………………………………………………………………17

不同酒精濃度和不同酒精倍數(shù)對(duì)灰樹花265多糖提取的影響

摘要：為了篩選出一種比較準(zhǔn)確、方便的多糖的提取方法, 本論文采用灰樹花265菌株發(fā)酵液，用四種不同酒精濃度為60%、70%、80%、90%分別進(jìn)行2倍、4倍、6倍、8倍不同酒精倍數(shù)醇沉正交實(shí)驗(yàn)，得到16個(gè)醇沉和醇溶提取物，利用硫酸-蒽酮法測(cè)定提取物的糖含量。在不同酒精濃度比較試驗(yàn)中，結(jié)果顯示：酒精濃度為70%時(shí)，測(cè)得糖含量較大，因此認(rèn)為酒精濃度應(yīng)該選取80%。在不同酒精倍數(shù)比較試驗(yàn)中，結(jié)果顯示：酒精倍數(shù)在2- 4之間隨著倍數(shù)的增大所測(cè)得糖含量逐漸增大，酒精倍數(shù)為4倍之后糖含量下降，酒精倍數(shù)為4倍時(shí)，測(cè)得糖含量最大，因此應(yīng)選取4倍。
關(guān)鍵詞：灰樹花；酒精提??；蒽酮-比色法；糖含量

Different alcohol concentration of alcohol and different times of Extraction of the impact of polysaccharide
 
  
Abstract: In order to select a more accurate and convenient method of extracting the polysaccharide, the paper used Grifola frondosa 265 strain broth, with four different alcohol concentration of 60% and 70%, 80% and 90% respectively 2 Times, four times, six times, eight times the multiples of different alcohol-alcohol orthogonal experiment, received 16-and alcohol extract of Alcohol, using sulfuric acid - anthrone-Determination extract of the sugar content. Comparing the different alcohol concentration test results showed that: alcohol concentration of 70 percent, measured higher sugar content, so that the concentration of alcohol should select 80%. At different times compared alcohol test results showed that: alcohol in multiples of 2 - 4 with multiples between the increase measured by the sugar content is gradually increasing, multiples of alcohol for four times after the decline in sugar content, alcohol multiples of four times when , The largest measured sugar content and therefore should be selected four times.
Key words: Grifola frondosa; Alcohol extraction; Anthrone-- colorimetry; Sugar content