小麥硫轉(zhuǎn)運(yùn)蛋白基因RT-PCR半定量檢測(cè)體系的建立
14頁(yè) 1.3萬(wàn)余字，包含完整畢業(yè)設(shè)計(jì)及答辯ppt
[摘要] 應(yīng)用RT-PCR技術(shù)， 以Tubulin為內(nèi)參基因，小麥硫轉(zhuǎn)運(yùn)蛋白基因(Sul)為檢測(cè)基因，優(yōu)化Mg2+濃度、退火溫度、循環(huán)次數(shù)、引物濃度和Taq酶濃度，獲得適宜的PCR反應(yīng)參數(shù)，建立一個(gè)相對(duì)穩(wěn)定、準(zhǔn)確、特異的檢測(cè)Sul表達(dá)量的半定量體系，為進(jìn)一步研究小麥硫轉(zhuǎn)運(yùn)蛋白表達(dá)調(diào)節(jié)機(jī)制奠定技術(shù)基礎(chǔ)。

[關(guān)鍵詞] 小麥；Tubulin；硫轉(zhuǎn)運(yùn)蛋白基因；RT-PCR；半定量PCR


To Establish a Method of Semiquantitative Polymerase Chain Reaction for Detecting Sul Gene Expression

Abstract To establish a method of semiquantitative polymerase chain reaction for detecting Sulphate transporter gene expression in wheat roots，we established a semiquantitative PCR by putting primer of Tubulin served as internal reference gene and primer of target gene in the same conditions and optimizing experiment parameters for PCR such as the concentration of Mg2+ and cycle times etc. This method is usable to test the expressive level of Sul gene in the wheat roots.

Key words：wheat；Sulphate transporter；RT-PCR；semiquantitative PCR







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