Bt抗蟲棉的研究報(bào)告

本文共有18頁，7600余字；

摘要：

為檢測Bt抗蟲棉棉籽及棉籽油對哺乳動物的誘變活性，以評價(jià)轉(zhuǎn)Bt基因抗蟲棉的安全性，本實(shí)驗(yàn)以環(huán)磷酰胺作為陽性誘變劑，以普通棉的棉籽及棉籽油作為對照物，同時(shí)設(shè)陰性對照組，以成年封閉群昆明種雄性小鼠為實(shí)驗(yàn)材料，將棉籽粉或棉籽油摻入飼料中飼喂小鼠，檢測小鼠骨髓嗜多染紅細(xì)胞（Polychromatic erythrocytes , PCE）微核率（Micronucleus frequency, MN‰）和精子畸變率（Sperm malformation rate）。實(shí)驗(yàn)結(jié)果顯示，飼喂棉籽及棉籽油飼料的實(shí)驗(yàn)組的微核率與陰性對照組相比，大多數(shù)P>0.05，無顯著性差異，有四組P<0.05，但它們的微核率比陰性對照組小，即這四組的微核率顯著小于陰性對照組；與陽性對照組相比，P<0.001，都有極顯著差異。上述實(shí)驗(yàn)組的精子畸變率與陰性對照組相比，P>0.05，無顯著性差異；與陽性對照組相比，P<0.05，有顯著性差異。結(jié)果表明：在本實(shí)驗(yàn)條件下，Bt抗蟲棉與普通棉的棉籽及棉籽油對小鼠體細(xì)胞和性細(xì)胞均未顯示出誘變活性，且沒有劑量效應(yīng)。

關(guān)鍵詞：Bt抗蟲棉 棉籽 棉籽油 嗜多染紅細(xì)胞微核率 精子畸變率

Study on Mutagenicity of Cotton Seed and Cotton Seed Oil
of Bt-transgenic Cottons

Abstract

To evaluate the safety of insect resistant cotton plants,which express a Bacillus thuringiensis protein,the mutagenicity of cotton seed and cotton seed oil on mammals was examined.The raw cotton seed and oil was mixed into mice’ diet. Using cyclophosphamide as positive control,non-modified cotton seed and cotton seed oil as controls, this study tested the micronucleus frequency in mice bone marrow polychromatic erythrocytes and the sperm malformation rate.The results showed that there’s no significant difference between most of the seed groups,oil groups and the negative control(P>0.05) or that the micronucleus frequency of the seed and oil groups is significantly lower than the negative control(P<0.05).On the other hand,there existed significant difference between the seed groups,oil groups and the positive control (micronucleus test P<0.001,sperm malformation test P<0.05).

目錄


一、前言.......................................................................................1
二、材料和方法..........................................................................3
2.1 材料....................................................................................3
2.2 方法....................................................................................3
2.2.1 實(shí)驗(yàn)分組.....................................................................3
2.2.2 實(shí)驗(yàn)處理.....................................................................3
2.2.3 活體小鼠微核試驗(yàn)..........................................................3
2.2.4 活體小鼠精子畸形試驗(yàn)...................................................4
三、結(jié)果.......................................................................................4
3.1 微核試驗(yàn)............................................................................5
3.2 精子畸形試驗(yàn)....................................................................6
3.3 棉籽及棉籽油對小鼠體重增長的影響...........................8
四、討論.....................................................................................13
五、小結(jié).....................................................................................14
六、參考文獻(xiàn).............................................................................15
致謝


六 參考文獻(xiàn)


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