TQ0701對大鼠腦缺血再灌注損傷的保護作用

目錄
摘要 1
前言 3
第一章 材料 6
1.1 藥物和試劑 6
1.2 主要儀器 7
1.3 動物 7
第二章 方法 8
2.1 TQ0701iv對局灶性腦缺血大鼠的神經(jīng)功能和腦組織梗塞率的影響 8
2.2 TQ0701iv對局灶性腦缺血大鼠腦組織學的影響 9
2.3 統(tǒng)計學處理 9
第三章 結果 10
3.1 TQ0701對局灶性腦缺血大鼠的神經(jīng)功能和腦組織梗塞率的影響 10
3.2 TQ0701對局灶性腦缺血大鼠腦組織學的影響 10
第四章 討論 12
參考文獻 14
致謝 16

摘要：目的: 探討新型的化合物TQ0701對大鼠腦缺血再灌注損傷保護作用的有效性，從而為該化合物的機制研究以及臨床應用開發(fā)提供相關實驗數(shù)據(jù)和實驗思路。
方法: 將60只雄性SD大鼠隨機分為假手術組、模型組、陽性對照組（依達拉奉3mg/kg）、TQ0701低劑量組(1.5mg/kg)、中劑量組(3mg/kg)、高劑量組(6mg/kg)各10只。假手術組僅進行手術而不造成缺血狀態(tài)，其余各組均采用Longa線栓法制備大鼠MCAO模型，在缺血2h后進行再灌注。給藥組和陽性對照組分別在缺血前30min以及再灌注0、2h尾靜脈注射TQ0701和依達拉奉，假手術組和模型組則給予等量的0.9%氯化鈉溶液。再灌注24h后觀察大鼠神經(jīng)功能評分、腦組織梗塞百分率和病理組織學的改變。
結果: 模型組大鼠的神經(jīng)功能評分和腦組織梗塞百分率同假手術組、陽性對照組以及TQ0701三個劑量組相比有顯著差異，陽性對照組與TQ0701三個劑量組相比未見顯著差異。同時電鏡觀察結果也發(fā)現(xiàn)TQ0701三個劑量組及陽性對照組與模型組相比神經(jīng)細胞病理改變較輕。
結論：化合物TQ0701對急性腦梗死有明顯的保護作用。
關鍵詞：TQ0701；腦缺血再灌注；依達拉奉；


Protective effects of TQ0701 on cerebral ischemic reperfusion injury in rats
Abstract: Aim: To investigate the effects of TQ0701 by dynamic changes of infarct volumes and neurological deficit scores on local cerebral ischemic-reperfusion injury in rats. Method: Male SD rats were randomly divided into sham-operated control group (n=10), normal saline control groups (n=10), edaravone-treated group (3mg/kg) (n=10) and TXL-treated groups (6mg/kg, 3mg/kg and 1.5mg/kg) (n=10 each). Animals in the latter four groups were subjected to transient focal ischemia by the middle cerebral artery occlusion (MCAO) for 120 minutes. The rats were pretreated with normal saline or TXL 30min before ischemic and 0min, 2hours after reperfusion. After 24hours of reperfusion, infarct volumes were determined by 2, 3, 5-triphenyltetrazolium chloride (TTC) and neurological scores were investigated. Results: Infarct volumes in TQ0701-treated groups were significantly decreased compared with those in control groups at 24 after MCAO, and the neurological deficit scores in TQ0701-treated groups were synchronously improved. Conclusion: These results show that treatment with TQ0701 can attenuate brain injury and TQ0701 may be a potential neuroprotective agent in cerebral ischemia-reperfusion.
Key words: TQ0701; cerebral ischemic reperfusion; edaravone