分 子 克 隆
Molecular Cloning

頁數(shù) 10 字?jǐn)?shù) 6949

摘要：分子克隆技術(shù)是指來自不同生物的基因用有自主復(fù)制能力的載體DNA在體外人工連接，構(gòu)建成新的重組DNA，然后送入受體生物中去表達(dá)，從而產(chǎn)生遺傳物質(zhì)狀態(tài)的轉(zhuǎn)移和重新組合。它包括質(zhì)粒載體的抽提，外源DNA的準(zhǔn)備，限制性內(nèi)切酶酶切，連接及感受態(tài)細(xì)胞的制備，連接產(chǎn)物轉(zhuǎn)化以及陽性克隆因子的鑒定和驗(yàn)證等。

關(guān)鍵詞：質(zhì)粒,感受態(tài)細(xì)胞,轉(zhuǎn)化子,PCR

Abstract: The molecule cloning technology is the different creature’s gene which have independence replication ability carry the DNA to go to the expression in outside work link, set up new reorganization DNA, then sent into suffer living creature, then heredity material transfer with the appearance with combine afresh. It includes the quality a take out for carrying lifts, the preparation of the outside source DNA, the enzyme slices, linking and the preparation of competent cell, linking outcome conversion and masculine cloning factors authenticate with verification etc.
Keywords: plasmid competence cell transformation factor
Polymerase chain reaction
參考文獻(xiàn)：
[1]《分子克隆技術(shù)》，實(shí)驗(yàn)操作手冊 ，李香花、 吳昌銀，華中農(nóng)業(yè)大學(xué)，2004
[2]《基因操作原理》，孫明，華中農(nóng)業(yè)大學(xué)，2004
[3]《分子遺傳學(xué)》，孫乃嗯，孫東序，南京大學(xué)出版社，1999
[4]《分子克隆實(shí)驗(yàn)指南》科學(xué)出版社，1995
[5]《生物化學(xué)》，王鏡巖，朱圣庚，徐長法，高等教育出版社，2002