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摘要

    耐高溫a一淀粉酶是目前最重要的工業(yè)酶制劑之一，約占酶制劑市場(chǎng)25%的比例。耐高溫a一淀粉酶能在較高溫度下能夠有效快速的分解淀粉，被廣泛應(yīng)用于紡織退漿、發(fā)酵、制糖等高溫條件下生產(chǎn)工業(yè)?？莶菅勘U菌是國(guó)內(nèi)生產(chǎn)耐高溫a一淀粉酶的代表菌株之一。

    為了獲得較高的酶活和產(chǎn)量，在實(shí)驗(yàn)中,對(duì)耐高溫a一淀粉酶產(chǎn)生菌枯草芽孢桿菌的發(fā)酵培養(yǎng)條件進(jìn)行了優(yōu)化.綜合考慮了所有影響酶活的因素，并對(duì)培養(yǎng)條件進(jìn)行了統(tǒng)計(jì)分析，得到產(chǎn)耐高溫a一淀粉酶的最佳培養(yǎng)條件。結(jié)果表明:優(yōu)化培養(yǎng)基的組成(ZoomL)為玉米粉25，豆粉25，玉米漿10mL/L，CaCL2 4 Na2HPO4 3,檸檬酸鈉 3，PH6.8~7.0.該營(yíng)養(yǎng)條件下的酶活為2329u’mL一，，約是培養(yǎng)基優(yōu)化前酶活的12.6倍，酶活有了顯著的提高

 

關(guān)鍵詞:a一淀粉酶，耐高溫，枯草芽抱桿菌.

Annual output of 10000 tons of high temperatureα- amylase fermentation plant design

ABSTRACT

Thermostable a-amysle, catalyzing hydrolysis of starch at high temperatures,is one of the most important .It is widely applied in desinzing,sugar and fermentation industry.bacillus subtilis is one of the industrial strains,which secretes thermostable a-amylase at high level

In the study ,the optimum medium for Bacillus subtilis .On the basis of single factor experiments,the prime factors affecting enzyme activity were selected.And then,the factors were optimized by response surface analysisThe result shows that the optimal medium composition was as follows:Corn flour25,Soybean meal25,Corn syrup 10mL/L, CaCL2 4, Na2HPO4 3,Sodium citrate3,PH6.8~7.0.Enzyme activity under the optimal culture conditions 2329 u/Ml.which is 12.6 fold of the orignai medium,this study has a significant advancement.The experimental data under various have validated the theoretical values

KEY WORDS:a一amylase，thermostable，Bacillus subtilis

目錄

引言························································· 1

第一章 緒論·················································  3

1.1 高溫α-淀粉酶·············································3

1.2 高溫α-淀粉酶的產(chǎn)生菌的生物學(xué)特征·························3

1.3 高溫α-淀粉酶的國(guó)內(nèi)外研究概況·····························3

1.3.1 高溫α-淀粉酶的國(guó)外研究概況·····························3

1.3.2 耐高溫α-淀粉酶的國(guó)內(nèi)研究·······························4

1.4 高溫淀粉酶的應(yīng)用 ·········································5

1.4.1 紡織物退漿············································· 6

1.4.2 酒精生產(chǎn)··············································· 6

1.4.3 淀粉糖生產(chǎn)··············································6

第2章 生產(chǎn)工藝流程···········································8

2.1 生產(chǎn)方法··················································8

2.2 工藝流程··················································8

2.3 操作工藝··················································8

2.3.1原料的處理··············································8

2.3.2 發(fā)酵工序················································8

第3章 發(fā)酵車間設(shè)備設(shè)計(jì)與選型·································9

3.1發(fā)酵罐的選型···············································9

3.1.1發(fā)酵罐容積與臺(tái)數(shù)的確定···································9

3.1.2主要尺寸的計(jì)算·········································· 9

3.1.3 發(fā)酵罐冷卻面積的計(jì)算····································10

3.1.4發(fā)酵罐攪拌器的設(shè)計(jì)······································10

3.1.5 發(fā)酵罐設(shè)備結(jié)構(gòu)的工藝設(shè)計(jì)································11

3.1.6發(fā)酵罐設(shè)備材料的選擇····································13

3.1.7發(fā)酵罐壁厚的計(jì)算········································13

3.1.8發(fā)酵罐接管設(shè)計(jì)··········································13

3.1.9發(fā)酵罐支座的選擇······································· 14

3.2發(fā)酵罐的選型··············································14

3.2.1種子罐容積和數(shù)量的選擇··································14

3.2.2 種子罐主要尺寸的確定····································14

3.2.3 種子罐型號(hào)的確定········································15

第四章 工藝計(jì)算書·············································16

4.1 物料衡算··················································16

4.1.1工藝技術(shù)指標(biāo)及基礎(chǔ)數(shù)據(jù)···································16

4.1.2 原料消耗計(jì)算（基準(zhǔn)：一噸成品高溫淀粉酶）··················16

4.1.2.1 所需發(fā)酵罐數(shù)量·········································16

4.1.2.2 總物料衡算·············································17

4.1.3 接種量···················································17

4.1.4 成品高溫淀粉酶···········································17

4.2 熱量衡算···················································18

4.2.1液化熱平衡計(jì)算············································18

4.2.2 發(fā)酵過程中的蒸汽耗量的計(jì)算·······························18

4.2.3 發(fā)酵過程中的冷卻水耗量計(jì)算·······························19

4.2.4 發(fā)酵過程中的無菌空氣耗用量的計(jì)算·························20

第5章  全廠及車間布置·········································21

第六章 “三廢”處理············································23

第七章 經(jīng)濟(jì)概算················································24

7.1 投資概算···················································24

7.1.1用于建筑工程的各項(xiàng)費(fèi)用···································24

7.2 利潤(rùn)、投資回收期計(jì)算·······································24

7.2.1利潤(rùn)·····················································24

7.2.2 投資回收期···············································24

7.3 小結(jié)·······················································24

結(jié)論與展望·····················································25

致謝···························································26

參考文獻(xiàn)·······················································27