紅掌組培快繁過程中污染原因的分析及對策探討

摘 要

紅掌（拉丁名：Spathiphyllum floribundum），亦叫安祖花，紅鶴芋等，天南星科火燭屬。其花色艷麗，花莖挺拔，葉型翠綠美觀，是當(dāng)今世界著名的切花和盆栽花卉之一，市場潛力大，具有極高的觀賞價(jià)值和經(jīng)濟(jì)價(jià)值。紅掌的繁殖方法通常采用分株繁殖、扦插繁殖和組織培養(yǎng)繁殖。但分株繁殖、扦插繁殖很難滿足當(dāng)前市場的需求，組織培養(yǎng)是紅掌快速繁殖的有效途徑。由于組織培養(yǎng)中經(jīng)常出現(xiàn)不同程度的污染問題，造成很大的損失，因此控制污染是植物組織培養(yǎng)苗工廠化生產(chǎn)中重要的技術(shù)環(huán)節(jié)。本文從滅菌方法、內(nèi)源性污染、繼代培養(yǎng)中污染防治等方面，進(jìn)行了紅掌組培快繁過程中污染原因的分析及對策的探討。

關(guān)鍵詞：紅掌，組織培養(yǎng)，滅菌，污染，防治
 
ANDRAEANUM DURING TISSUE CULTURE ANALYSIS OF THE CAUSES OF POLLUTION AND RELATED COUNTERMEASURES

ABSTRACT

Anthurium, also called Andrew Flower, lines such as flamingo, a Fire Araceae. Its bright colors, tall and straight stem, green leaf-type appearance, are well-known in today's world of cut and potted flowers, one large market potential, with high ornamental value and economic value. Flower andraeanum breeding methods commonly used ramets propagation, cutting propagation and tissue cultur e propagation. However, ramets propagation, cutting propagation is difficult to meet current market demand, tissue culture andraeanum Flower Express are an effective way of breeding. Tissue culture because of the frequent occurrence of various degrees of pollution, resulting in great loss, so control of pollution are industrial plant tissue culture vaccine production technology in the important aspect. This article from the sterilization method, endogenous pollution subculture in such areas as pollution prevention, to discuss how to minimize the emergence of tissue in the pollution problems.

KEY WORDS: Anthurium，tissue culture， sterilization， pollution，prevention and treatment
 
目　錄

前　言 1
第1章 培養(yǎng)基的滅菌 2
1.1一般培養(yǎng)基的滅菌 2
1.2不耐熱物質(zhì)的滅菌 2
第2章 外植體材料的消毒與滅菌 4
2.1外植體的消毒與滅菌 4
第3章 接種前的準(zhǔn)備工作 5
3.1器皿、工具的滅菌 5
3.1.1器皿與金屬器械的滅菌 5
3.1.2布質(zhì)制品的滅菌 5
3.2培養(yǎng)室的滅菌 5
3.3無菌操作和無菌操作技術(shù) 5
第4章 紅掌誘導(dǎo)與繼代培養(yǎng)過程中的污染 7
第5章 污染的原因和預(yù)防措施 8
5.1通常污染 8
5.2內(nèi)源性污染 8
5.3污染原因判斷及污染苗搶救 8
5.4預(yù)防方法和措施 9
5.4.1基本方法 9
5.4.2經(jīng)常檢查消毒鍋的滅菌質(zhì)量 9
5.4.3檢查培養(yǎng)容器是否存在問題 10
5.4.4繼代培養(yǎng)中污染的控制 10
5.4.5減少培養(yǎng)基中的有機(jī)成分 10
結(jié)　論 11
謝  辭 12
參考文獻(xiàn) 13
附　錄 15
外文資料翻譯 18