題 目：熒光性雙層膜的制備及其在汞離子檢測(cè)中的應(yīng)用
院 系：材料科學(xué)與工程學(xué)院
專 業(yè)：環(huán)境工程
目錄
一 前言 5
二 實(shí)驗(yàn)部分 6
2.1 實(shí)驗(yàn)儀器與試劑 6
2.1.1 儀器 6
2.1.2 試劑 6
2.2 實(shí)驗(yàn)內(nèi)容 7
2.2.1 自組裝膜的制備 7
2.2.1.1 組裝機(jī)理 7
2.2.1.2 Cys/Au的制備 7
2.2.1.3 HOX/Cys/Au自組裝膜的制備 7
2.2.2 8-羥基喹啉鋁（8-羥基喹啉汞）的合成 8
2.2.3 Al(OX)3/Cys/Au的制備 8
2.2.4 自組裝膜熒光光譜測(cè)定 8
2.2.5 電化學(xué)實(shí)驗(yàn) 9
2.2.6 膜的結(jié)構(gòu)表征 9
三 結(jié)果與討論 9
3.1 8-羥基喹啉和8-羥基喹啉鋁溶液溶劑、激發(fā)波長(zhǎng)和pH的選擇 9
3.1.1 溶劑的選擇 9
3.1.2 8-羥基喹啉和8-羥基喹啉鋁溶液的激發(fā)波長(zhǎng)的選擇 10
3.1.3 8-羥基喹啉和8-羥基喹啉鋁溶液pH值的選擇 10
3.2 自組裝膜優(yōu)化條件的選擇 11
3.2.1.1 半胱胺酸在金表面上自組裝的電化學(xué)表征 11
3.2.1.2 組裝DL－半胱氨酸濃度的選擇 12
3.2.1.3 組裝DL－半胱氨酸時(shí)間的選擇 12
3.2.1.4 組裝DL－半胱氨酸pH值的選擇 13
3.2.2 Cys/Au電極酸化時(shí)間和濃度的選擇 13
3.2.3 8－羥基喹琳熒光物質(zhì)及其金屬螯合物的組裝 13
3.2.3.1 確定熒光物質(zhì)的激發(fā)波長(zhǎng) 13
3.2.3.2 浸泡時(shí)間與熒光物質(zhì)濃度的選擇 14
3.2.3.3 pH值的選擇 14
3.3 自組裝雙層膜的熒光光譜 15
3.4 自組裝膜的表征 16
3.4.1 電極熒光光譜表征 16
3.4.2 膜的結(jié)構(gòu)表征 16
3.5 8－羥基喹啉鋁、8－羥基喹啉溶液及自組裝膜穩(wěn)定性能的比較 17
3.5.1 溶液熒光強(qiáng)度的比較 17
3.5.2 穩(wěn)定性的比較 17
3.6 Hg2+的熒光傳感分析 18
3.6.1 汞離子的熒光傳感的分析特性 18
四 結(jié)論 20
五 參考文獻(xiàn) 20

Abstract

Self-assembled monolayer(SAMs) can be designed according to our demand in molecular level，It has many merits which don’t exit in other methods. Now SAMs has been extensive applied in electroanalytical chemistry. But it is few reported about its application in fluorescence analysis. Fluorescence analysis is an important and effective spectrum analysis，If we can combine combine SAMs technology with fluorescence analytical method and develop another interface fluorescence analytical method，It was effective in diminishing the sample volume with a view of on-chip analysis，therefore has promising prospects in fluorescence analysis and chemical sensor.
The fluorescence intensity of 8-hydroxyquinoline is low，in order to improve its fluorescence intensity , in this paper we synthesized fluorescence reagent 8-hydroxyquinoline aluminum(Al(OX)3 , It is a very significant electroluminscent material and has attracted greated attention both from fundamental and potential application point of view .
In this experiment , The self-assembled bilayer membrane Al(OX)3/Cys/Au was fabricated via electrostatic action with DL-cysteine(Cys)monolayers on gold,surface. in order to characterized its structure , we make use of cyclic voltammetry(CV)in combination with X-ray Photoelectron spectra(XPS) .
It was found that the self-assembled bilayer membrane Al(OX)3/Cys/Au has good selectivity for Hg2+, so it can be applied to the detection of mercury ion. Moreover, we found that fluorescent signal of Al(OX)3/Cys/Au can be quenched by Hg2+.

Keyword: fluorescence self-assembled bilayer membrane
8-hydroxyquinoline mercury ion