魚腥草濃縮丸的制備

魚腥草濃縮丸的制備
摘    要
魚腥草（Herba houttuyniae）別名紫蕺、臭豬巢、側(cè)耳根、豬鼻拱，為三百草科多年生草本植物蕺菜的干燥水上部分，產(chǎn)于我國(guó)長(zhǎng)江流域以南各省。魚腥草的藥理活性表明，魚腥草具有提高機(jī)體免疫力、抗菌、抗病毒、利尿等臨床應(yīng)用。然而傳統(tǒng)的魚腥草制劑多以片劑為主，不適宜幼兒等特定人群服用，而且影響崩解和溶出，不利于藥物的吸收，研究魚腥草濃縮丸劑型有利于改善上述不利因素，具有很好的開發(fā)前景。
本文分別以水、70%乙醇作為提取溶劑，采用熱回流提取魚腥草內(nèi)的藥用活性物質(zhì)總黃酮，進(jìn)行驗(yàn)證比較，選擇最佳提取方法是70%乙醇溶劑提取。然后應(yīng)用旋轉(zhuǎn)蒸發(fā)儀對(duì)提取液進(jìn)行濃縮，經(jīng)過濃縮丸處方摸索，通過正交試驗(yàn)確定最佳處方工藝，主藥、蜂蜜、填充劑的質(zhì)量比為1:1.5:2.5，加蜜溫度60℃，并且應(yīng)用紫外分光光度計(jì)、崩解時(shí)限儀、溶出儀器進(jìn)行了檢測(cè)試驗(yàn)。實(shí)驗(yàn)結(jié)果表明：崩解時(shí)間為29 min，符合2010版一部藥典各項(xiàng)標(biāo)準(zhǔn)。

關(guān)鍵詞  魚腥草；濃縮丸；質(zhì)量控制；制備工藝

Preparation of herba houttuyniae condensed pill
Abstract
Herba houttuyniae Synonyms is named purple Houttuynia cordata Thunb、smelly pig nest、ear root、pig nose arch. It is the dry part of the Houttuyniae cordata that belongs to saururaceae. It is produced in the provinces south of Yangtze River in China. Pharmacological activity of Houttuynia indicates that Houttuynia has increased immunity, antibacterial, antiviral, diuretic and other clinical applications. However, tablet of Houttuynia is mainly form in the traditional formulations, and is not suitable for children as a specific group of people with using them, but also it affects the disintegration and dissolution, is not conducive to drug absorption. Concentrated pill will help to improve the above-mentioned negative factors, with good development prospects.
In This experiment it was studied by making water and 70% ethanol as extraction solvent and refluxing to extract the total flavonoids of medicinal active substance within Houttuynia. This paper determined the optimal extraction proce. The results were shown that: the best extraction solvent was 70% ethanol. Afterwards the Rotary Evaporator was applied by extracting and going on with enrichment, the prescriptions of concentrated pill about Houttuynia was groped by the orthogonal test to determine the optimum formulation process, the main drug, honey and filler mass ratio was 1:1.5:2.5, temperature by adding honey was 60 ℃. This word used ultraviolet spectrophotometer, disintegration time apparatus, dissolution test apparatus to go on testing. The results showed that: the disintegration time was 29 min and was in accordance with the standard 2010 version of Pharmacopoeia of China.
Keywords: Herba houttuyniae;condensed pill;quality control; preparation technology
 
目  錄
摘  要 I
Abstract II
第1章 緒  論 1
1.1 課題研究背景 1
1.2 相關(guān)知識(shí)簡(jiǎn)介 1
1.2.1 魚腥草簡(jiǎn)介 1
1.2.2 濃縮丸簡(jiǎn)介 3
1.3 濃縮丸研究進(jìn)展 5
1.3.1 濃縮丸的優(yōu)點(diǎn) 5
1.3.2 濃縮丸的特點(diǎn) 6
1.3.3 濃縮丸存在的問題 7
1.3.4 前景及展望 7
1.4 本實(shí)驗(yàn)研究主要內(nèi)容 7
第2章 總黃酮的提取 8
2.1 試劑與儀器 8
2.1.1 試劑 8
2.1.2 儀器 8
2.2 實(shí)驗(yàn)部分 9
2.2.1 總黃酮的提取方法比較 9
2.2.2 總黃酮的含量測(cè)定 9
2.2.3 方法學(xué)考察 10
2.3 討論 11
2.4 本章小結(jié) 12
第3章 濃縮丸的制備 13
3.1 出膏率的測(cè)定 13
3.2 處方的確定 13
3.2.1 單因素考察 13
3.2.2 正交實(shí)驗(yàn)確定最佳處方工藝 15
3.2.3 最佳處方工藝驗(yàn)證 16
3.2.4 本實(shí)驗(yàn)濃縮丸處方 16
3.3 濃縮丸質(zhì)量控制檢測(cè) 17
3.3.1 崩解時(shí)限檢測(cè) 17
3.3.2 溶出度檢測(cè) 17
3.4 討論 19
3.5 本章小結(jié) 20
結(jié)  論 21
致  謝 22
參考文獻(xiàn) 23
附錄 A 25
附錄 B 30