畢業(yè)論文 胃康靈膠囊質(zhì)量標(biāo)準(zhǔn)研究.doc
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畢業(yè)論文 胃康靈膠囊質(zhì)量標(biāo)準(zhǔn)研究,目錄中文摘要1英文摘要21實(shí)驗(yàn)材料31.1藥品與對(duì)照品31.2儀器與試劑32薄層鑒別32.1甘草的薄層鑒別32.2白及的薄層鑒別42.3延胡索的薄層鑒別42.4三七的薄層鑒別53 質(zhì)量標(biāo)準(zhǔn)研究63.1色譜條件的選擇63.2標(biāo)準(zhǔn)曲線的繪制與線性范圍考察83.3供試品溶液的制備93.4陰性對(duì)照實(shí)驗(yàn)103.5穩(wěn)定性實(shí)驗(yàn)123...
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目 錄
中文摘要…………………………………………………………………………………1
英文摘要…………………………………………………………………………………2
1實(shí)驗(yàn)材料………………………………………………………………………………3
1.1藥品與對(duì)照品………………………………………………………………………3
1.2儀器與試劑…………………………………………………………………………3
2薄層鑒別………………………………………………………………………………3
2.1甘草的薄層鑒別……………………………………………………………………3
2.2白及的薄層鑒別……………………………………………………………………4
2.3延胡索的薄層鑒別…………………………………………………………………4
2.4三七的薄層鑒別……………………………………………………………………5
3 質(zhì)量標(biāo)準(zhǔn)研究…………………………………………………………………………6
3.1色譜條件的選擇……………………………………………………………………6
3.2標(biāo)準(zhǔn)曲線的繪制與線性范圍考察…………………………………………………8
3.3供試品溶液的制備…………………………………………………………………9
3.4陰性對(duì)照實(shí)驗(yàn)………………………………………………………………………10
3.5穩(wěn)定性實(shí)驗(yàn)…………………………………………………………………………12
3.6重現(xiàn)性試驗(yàn)…………………………………………………………………………12
3.7加樣回收率試驗(yàn)……………………………………………………………………13
4樣品測(cè)定………………………………………………………………………………13
5 結(jié)果與討論……………………………………………………………………………14
參考文獻(xiàn)…………………………………………………………………………………15
致謝………………………………………………………………………………………16
綜述………………………………………………………………………………………17
中文摘要
目的：研究并確定胃康靈膠囊的質(zhì)量標(biāo)準(zhǔn).
方法:采用TLC法及HPLC法對(duì)胃康靈膠囊進(jìn)行鑒別和含量檢測(cè), 采用Diamonsil C18色譜柱:以乙腈-0.1%磷酸溶液(14:88)為流動(dòng)相: 流速:1.0ml/min: 柱溫:30℃: 檢測(cè)波長(zhǎng)為230nm
結(jié)果:按改進(jìn)后的方法進(jìn)行實(shí)驗(yàn)，雜質(zhì)峰干擾較少，芍藥苷分離度及拖尾因子較好，而且大大縮短了進(jìn)樣測(cè)試時(shí)間。同時(shí)在TLC鑒別中排除了陰性是否有干擾.
結(jié)論:TCL鑒別中陰性無(wú)干擾，含量測(cè)定改進(jìn)方法后實(shí)驗(yàn)較滿意，可以確定為其質(zhì)量標(biāo)準(zhǔn)
關(guān)鍵詞： 質(zhì)量標(biāo)準(zhǔn)；薄層鑒別；高效液相
ABSTRACT
Objective: To study and determine Weikangling capsule’s quality standards. Methods: TLC method and HPLC method were used for identification and testing of Weikangling capsule ,using Diamonsil C18 column, acetonitrile -0.1% phosphoric acid (14 : 88) at a flow rate: 1.0 ml / min, temperature: 30 ℃, detection at 230 nm. Results: by improved of the method of experiment, less interference-impurities, paeoniflorin isolation and tailing factor Better, but also shortened the sample testing time.and there is no interference in the TLC identification. Conclusion: TCL experients have no negative interference in the identification, determination of improving the methods of more satisfactory, so we can to determine it to be quality standards.
Key word: quality standards；TLC；HPLC
中文摘要…………………………………………………………………………………1
英文摘要…………………………………………………………………………………2
1實(shí)驗(yàn)材料………………………………………………………………………………3
1.1藥品與對(duì)照品………………………………………………………………………3
1.2儀器與試劑…………………………………………………………………………3
2薄層鑒別………………………………………………………………………………3
2.1甘草的薄層鑒別……………………………………………………………………3
2.2白及的薄層鑒別……………………………………………………………………4
2.3延胡索的薄層鑒別…………………………………………………………………4
2.4三七的薄層鑒別……………………………………………………………………5
3 質(zhì)量標(biāo)準(zhǔn)研究…………………………………………………………………………6
3.1色譜條件的選擇……………………………………………………………………6
3.2標(biāo)準(zhǔn)曲線的繪制與線性范圍考察…………………………………………………8
3.3供試品溶液的制備…………………………………………………………………9
3.4陰性對(duì)照實(shí)驗(yàn)………………………………………………………………………10
3.5穩(wěn)定性實(shí)驗(yàn)…………………………………………………………………………12
3.6重現(xiàn)性試驗(yàn)…………………………………………………………………………12
3.7加樣回收率試驗(yàn)……………………………………………………………………13
4樣品測(cè)定………………………………………………………………………………13
5 結(jié)果與討論……………………………………………………………………………14
參考文獻(xiàn)…………………………………………………………………………………15
致謝………………………………………………………………………………………16
綜述………………………………………………………………………………………17
中文摘要
目的：研究并確定胃康靈膠囊的質(zhì)量標(biāo)準(zhǔn).
方法:采用TLC法及HPLC法對(duì)胃康靈膠囊進(jìn)行鑒別和含量檢測(cè), 采用Diamonsil C18色譜柱:以乙腈-0.1%磷酸溶液(14:88)為流動(dòng)相: 流速:1.0ml/min: 柱溫:30℃: 檢測(cè)波長(zhǎng)為230nm
結(jié)果:按改進(jìn)后的方法進(jìn)行實(shí)驗(yàn)，雜質(zhì)峰干擾較少，芍藥苷分離度及拖尾因子較好，而且大大縮短了進(jìn)樣測(cè)試時(shí)間。同時(shí)在TLC鑒別中排除了陰性是否有干擾.
結(jié)論:TCL鑒別中陰性無(wú)干擾，含量測(cè)定改進(jìn)方法后實(shí)驗(yàn)較滿意，可以確定為其質(zhì)量標(biāo)準(zhǔn)
關(guān)鍵詞： 質(zhì)量標(biāo)準(zhǔn)；薄層鑒別；高效液相
ABSTRACT
Objective: To study and determine Weikangling capsule’s quality standards. Methods: TLC method and HPLC method were used for identification and testing of Weikangling capsule ,using Diamonsil C18 column, acetonitrile -0.1% phosphoric acid (14 : 88) at a flow rate: 1.0 ml / min, temperature: 30 ℃, detection at 230 nm. Results: by improved of the method of experiment, less interference-impurities, paeoniflorin isolation and tailing factor Better, but also shortened the sample testing time.and there is no interference in the TLC identification. Conclusion: TCL experients have no negative interference in the identification, determination of improving the methods of more satisfactory, so we can to determine it to be quality standards.
Key word: quality standards；TLC；HPLC
