植物染色體制片技術(shù)的比較研究

頁數(shù) 3 字?jǐn)?shù) 2903

摘要：本文以韭蘭為材料，對植物染色體常規(guī)壓片，去壁低滲法制片技術(shù)、染色效果進(jìn)行了比較。實(shí)驗(yàn)表明：去壁低滲法獲得細(xì)胞和染色體都較分散，Giemsa染色使得背景與材料的對比度較大，便與觀察。不足之處在于去壁低滲過程中，時(shí)間特別不易掌握，耗材多，且成功率較低。染色體容易丟失、形態(tài)也不太理想。常規(guī)壓片耗材少，成功率較高，但不易做分帶制片。

關(guān)鍵詞 植物染色體,制片技術(shù),去壁低滲,常規(guī)壓片

This paper with Jiu orchid is material, for plant chromosome conventional sheeting, go to wall low seep legal system flat technology and dyeing effect compare . test show : go to wall low seep law get cell and chromosome scatter comparatively, Giemsa dyeing make background be greater, convenient with the contrast ratio of material with observe . is below is in in go to wall low seep process in, time do not be easy especially grasp , consumed material much, succeed just lead lower. chromosome lose easily , form also not quite ideal. conventional sheeting consumed material lose , successful rate higher, but do not be easy do minute take production
Key words:plant ;technique of making chromosome; way of ridding cell wall and putting low osmosis

[6] 王新民 再談植物染色體標(biāo)本制備的去壁、低滲法[J]