本文是本人的本科畢業(yè)論文，重要內容包括解磷菌株的篩選，以及對其各種不同發(fā)酵條件和參數(shù)下其解磷能力的研究。內容涉及微生物方面的各種實驗、分析和研究方法，詳見目錄，內容充實，圖文并茂，物有所值，歡迎下載

目    錄
1  引言 1
2  材料與方法 2
2.1 主要儀器設備 2
2.2 菌種的分離與純化 2
2.2.1 分離培養(yǎng)基 3
2.2.2 菌種分離 3
2.2.3 菌種純化 3
2.3 解磷能力的初步測定 4
2.4 不同條件對菌株的解磷能力的影響 4
2.4.1 測定原理與方法 4
2.4.2 不同培養(yǎng)時間對解磷能力的影響 5
2.4.3 不同碳源對解磷能力的影響 6
2.4.4 不同氮源對解磷能力的影響 6
2.4.5 不同的磷酸鈣量對解磷能力的影響 6
2.4.6 不同鹽濃度對解磷能力的影響 6
3  結果與分析 7
3.1 分解無機磷菌株的篩選結果 7
3.2 不同條件對解磷能力的影響 8
3.2.1 不同培養(yǎng)時間對菌株解磷能力的影響 8
3.2.2 不同碳源對解磷能力的影響 9
3.2.3 不同氮源對解磷能力的影響 10
3.2.4 不同的磷酸鈣量對解磷能力的影響 11
3.2.5 不同鹽濃度對解磷能力的影響 12
4  討論與結論 13
4.1 討論 13
4.2 結論 14
參考文獻 15
致謝 16
附錄1相關英文文獻 17
附錄2英文文獻中文譯文 24


摘    要
　　　本研究利用無機磷固體培養(yǎng)基，通過劃線分離的方法，從人工濕地中采集的樣品中篩選出具有分解無機磷能力的菌株共6株，通過比較D/d值初步確定解磷能力的大小，選擇D/d值較大的4株，采用不同碳源、不同氮源、不同磷酸鈣含量和不同鹽濃度等液體培養(yǎng)基，用鉬銻抗分光光度法測定各菌株對無機磷的分解能力，從而討論不同條件對其解磷能力的影響。研究結果表明：4個菌株最容易利用的碳源是葡萄糖，而最不容易利用的碳源是蔗糖，碳源種類對解磷能力影響較大；氮源種類對各菌株的解磷能力影響較小；磷酸鈣含量為1%時，4個菌株解磷能力最強，磷酸鈣含量為4%時解磷能力受到嚴重抑制；NaCl濃度為4%時，4個菌株對無機磷分解能力最強；當NaCl濃度達到10%，各菌株的解磷能力受到嚴重抑制。

關鍵詞：人工濕地；分解無機磷菌株；解磷能力

ABSTRACT

　　Using solid medium that contained inorganic phosphorus and the method of streak, 6 phosphate-solubilizing bacterial strains were isolated from the samples taken from artificial wetland. Through comparing the phosphate-solubilizing ability preliminarily, four strains that had larger D/d were studied. Using fluid medium that consisted of the different carbon source, nitrogen source, calcium phosphate content and concentration of NaCl, and Molybdenum antimony anti - spectrophotometric method discussed the influence that diffent conditions on phosphate-solubilizing ability. Some conclusions are obtained. firstly, glucose was the most easy to be used, and sucrose as carbon source was the most difficult to be used for the four strains, and carbon source was a major influence factor for phosphate-solubilizing ability. Secondly, nitrogen source hadn’t an obvious effect on phosphate-solubilizing ability for the four strains. Thirdly, when the calcium phosphate concentration was 1%, they had the largest phosphate-solubilizing capacity. When the calcium phosphate concentration was 4%, their phosphate-solubilizing capacity was restrained seriously. Fourthly, when the concentration of NaCl was 4%, they had the largest phosphate-solubilizing capacity. When the concentration of NaCl was 10%, their phosphate-solubilizing capacity was restrained seriously.

Key words: artificial wetland; decomposing inorganic phosphorus bacteria; phosphate-solubilizing ability