本文是本人的本科畢業(yè)論文，重要內(nèi)容包括解磷菌株的篩選，以及對(duì)其各種不同發(fā)酵條件和參數(shù)下其解磷能力的研究。內(nèi)容涉及微生物方面的各種實(shí)驗(yàn)、分析和研究方法，詳見目錄，內(nèi)容充實(shí)，圖文并茂，物有所值，歡迎下載

目    錄
1  引言 1
2  材料與方法 2
2.1 主要儀器設(shè)備 2
2.2 菌種的分離與純化 2
2.2.1 分離培養(yǎng)基 3
2.2.2 菌種分離 3
2.2.3 菌種純化 3
2.3 解磷能力的初步測(cè)定 4
2.4 不同條件對(duì)菌株的解磷能力的影響 4
2.4.1 測(cè)定原理與方法 4
2.4.2 不同培養(yǎng)時(shí)間對(duì)解磷能力的影響 5
2.4.3 不同碳源對(duì)解磷能力的影響 6
2.4.4 不同氮源對(duì)解磷能力的影響 6
2.4.5 不同的磷酸鈣量對(duì)解磷能力的影響 6
2.4.6 不同鹽濃度對(duì)解磷能力的影響 6
3  結(jié)果與分析 7
3.1 分解無(wú)機(jī)磷菌株的篩選結(jié)果 7
3.2 不同條件對(duì)解磷能力的影響 8
3.2.1 不同培養(yǎng)時(shí)間對(duì)菌株解磷能力的影響 8
3.2.2 不同碳源對(duì)解磷能力的影響 9
3.2.3 不同氮源對(duì)解磷能力的影響 10
3.2.4 不同的磷酸鈣量對(duì)解磷能力的影響 11
3.2.5 不同鹽濃度對(duì)解磷能力的影響 12
4  討論與結(jié)論 13
4.1 討論 13
4.2 結(jié)論 14
參考文獻(xiàn) 15
致謝 16
附錄1相關(guān)英文文獻(xiàn) 17
附錄2英文文獻(xiàn)中文譯文 24


摘    要
　　　本研究利用無(wú)機(jī)磷固體培養(yǎng)基，通過(guò)劃線分離的方法，從人工濕地中采集的樣品中篩選出具有分解無(wú)機(jī)磷能力的菌株共6株，通過(guò)比較D/d值初步確定解磷能力的大小，選擇D/d值較大的4株，采用不同碳源、不同氮源、不同磷酸鈣含量和不同鹽濃度等液體培養(yǎng)基，用鉬銻抗分光光度法測(cè)定各菌株對(duì)無(wú)機(jī)磷的分解能力，從而討論不同條件對(duì)其解磷能力的影響。研究結(jié)果表明：4個(gè)菌株最容易利用的碳源是葡萄糖，而最不容易利用的碳源是蔗糖，碳源種類對(duì)解磷能力影響較大；氮源種類對(duì)各菌株的解磷能力影響較??；磷酸鈣含量為1%時(shí)，4個(gè)菌株解磷能力最強(qiáng)，磷酸鈣含量為4%時(shí)解磷能力受到嚴(yán)重抑制；NaCl濃度為4%時(shí)，4個(gè)菌株對(duì)無(wú)機(jī)磷分解能力最強(qiáng)；當(dāng)NaCl濃度達(dá)到10%，各菌株的解磷能力受到嚴(yán)重抑制。

關(guān)鍵詞：人工濕地；分解無(wú)機(jī)磷菌株；解磷能力

ABSTRACT

　　Using solid medium that contained inorganic phosphorus and the method of streak, 6 phosphate-solubilizing bacterial strains were isolated from the samples taken from artificial wetland. Through comparing the phosphate-solubilizing ability preliminarily, four strains that had larger D/d were studied. Using fluid medium that consisted of the different carbon source, nitrogen source, calcium phosphate content and concentration of NaCl, and Molybdenum antimony anti - spectrophotometric method discussed the influence that diffent conditions on phosphate-solubilizing ability. Some conclusions are obtained. firstly, glucose was the most easy to be used, and sucrose as carbon source was the most difficult to be used for the four strains, and carbon source was a major influence factor for phosphate-solubilizing ability. Secondly, nitrogen source hadn’t an obvious effect on phosphate-solubilizing ability for the four strains. Thirdly, when the calcium phosphate concentration was 1%, they had the largest phosphate-solubilizing capacity. When the calcium phosphate concentration was 4%, their phosphate-solubilizing capacity was restrained seriously. Fourthly, when the concentration of NaCl was 4%, they had the largest phosphate-solubilizing capacity. When the concentration of NaCl was 10%, their phosphate-solubilizing capacity was restrained seriously.

Key words: artificial wetland; decomposing inorganic phosphorus bacteria; phosphate-solubilizing ability