HPLC測氨茶堿的血清藥物濃度

摘要：目的 為測定人血清中氨茶堿濃度建立一個(gè)準(zhǔn)確的高效液相色譜法，為臨床開展氨茶堿血清藥物濃度的監(jiān)測提供重要保障。實(shí)驗(yàn)方法 利用日本島津LC-20AT高效液相色譜儀來測定氨茶堿的血清藥物濃度，用規(guī)格為150mm*4.6mm*5μm的ODS-C18柱為色譜柱，采用 0.8mL/min的流速，15℃的柱溫，同時(shí)采用甲醇:水（22:78）為流動(dòng)相，檢測時(shí)波長為210nm，進(jìn)樣量為20μL。結(jié)果 獲得血樣處理辦法和色譜條件選擇方案，對所選方法的可行性進(jìn)行相關(guān)性試驗(yàn)，所得測定人血清中氨茶堿血清藥物濃度的色譜法的適用性得到證實(shí)，且在測定氨茶堿藥物濃度時(shí)得到了較為良好的線性關(guān)系，據(jù)數(shù)據(jù)顯示此方法檢測時(shí)可以得到的線性范圍在1.62μg/mL -37.8μg/mL（r＝0.999）之間，實(shí)驗(yàn)方法的回收率均>95％，得到的日內(nèi)和日間的精密度均＜4%（n＝5）。結(jié)論 在實(shí)驗(yàn)中探索得到的氨茶堿血清藥物濃度測定方法具有可行性，能準(zhǔn)確、靈敏的測出血清中氨茶堿的藥物濃度，同時(shí)具有精密、準(zhǔn)確、可多次重復(fù)的特點(diǎn)。

關(guān)鍵詞：血藥濃度；藥物相互作用；高效液相色譜法；氨茶堿

Method of Aminophylline concentration in serum by HPLC


Abstract: Objective Using RP-HPLC to establish an accurate means to measure the serum concentration of Aminophylline, to provide an important safeguard to carry out the monitoring of Aminophylline serum concentrations for the clinical. Experimental methods Use Shimadzu LC-20AT HPLC to measure the serum concentrations of Aminophylline, ODS-C18 column (150mm * 4.6mm * 5μm) column, and set the flow rate of 0.8mL/min, column temperature was 15 °C, using methanol: water (22:78) at the same time as the mobile phase, measuring wavelength 210nm, the injection volume is 20 ul. The experimental results From lots of experimental exploration we can get the Blood sample approach and Chromatographic conditions options. And the Correlation test about feasibility of the selected method Confirmed that the method is indeed suitable for the determination of Aminophylline serum drug concentration. And a relatively good linear relationship is obtained in the determination of the drug concentration of Aminophylline. According to the data show that this method of measuring can get the linear range 1.62μg/mL to 37.8μg/mL (r = 0.999) , the recovery rate of this method is more than 95%, and the intra-and inter precision is less than 4% (n = 5).Conclusions The measuring method of Aminophylline serum drug concentration in the experiment is feasible, it can accurately and sensitively measured the Aminophylline serum drug concentration, and it have the characters of good repeatability, high accuracy and so on
Keywords: Blood concentration；Drug Interactions；HPLC method；Aminophylline