目 錄
摘要 1
1 前言 3
1.1 環(huán)境內(nèi)分泌干擾物 3
1.2 碳糊電極的特點(diǎn)及應(yīng)用 4
1.3 循環(huán)伏安法的特點(diǎn)及應(yīng)用 4
1.4 生物樣品分析 5
2 實(shí)驗(yàn)部分 5
2.1 實(shí)驗(yàn)儀器及試劑 5
2.2 碳糊電極的制備 5
2.3 實(shí)驗(yàn)方法 6
3 結(jié)果與討論 6
3.1 底液的選擇 6
3.2 底液pH的影響 7
3.3 富集時間的影響 8
3.4 掃描速度的影響 8
3.5 線性范圍、校正曲線 9
3.6 電極的重現(xiàn)性、穩(wěn)定性 10
3.7 干擾實(shí)驗(yàn) 10
3.8 回收率及樣品的測定 11
4 結(jié)論 11
參考文獻(xiàn) 12
致謝 13
附錄 13






環(huán)境激素辛基酚的電化學(xué)檢測
化工80703 束林玲 指導(dǎo)教師：胡效亞 教授
摘要：本文研究了辛基酚在碳糊電極上的電化學(xué)性質(zhì)，在-0.2~1.2V的電位范圍內(nèi)進(jìn)行了循環(huán)伏安掃描，辛基酚在0.7V處有一明顯的氧化峰，根據(jù)該氧化峰的電流與濃度的關(guān)系建立了循環(huán)伏安法測定辛基酚的電化學(xué)分析方法。對辛基酚檢測方法的條件進(jìn)行了優(yōu)化。結(jié)果表明：測定的最佳底液是pH=5的磷酸鹽緩沖溶液PBS，掃描速率為100mV/s。辛基酚濃度與氧化峰電流在7.0×10-7~3.1×10-5mol/L范圍內(nèi)呈良好的線性關(guān)系，線性方程為：ipa (A)= 2.0×10-6+0.4598 c mol/L,線性相關(guān)系數(shù)r= 0.9966，該方法的檢測限為4.0×10-7 mol/L。用標(biāo)準(zhǔn)加入法對辛基酚樣品的回收率進(jìn)行測定，回收率范圍在96.5%～105.4% 之間，平均回收率為99.7%。將該方法用于鯽魚體內(nèi)辛基酚含量的測定取得較好的結(jié)果。該方法具有電極制作簡單，操作簡便，測定結(jié)果準(zhǔn)確等優(yōu)點(diǎn)。
關(guān)鍵詞：碳糊電極；辛基酚；循環(huán)伏安法；鯽魚

Electrochemical Detection of Environmental Hormone Octyl Phenol
Abstract: This paper had investigated the electrochemical properties of the octyl phenol in carbon paste electrodes, cyclic voltammetric scanning was proceeded in -0.2~1.2V potential range, meanwhile, octyl phenol was an obvious oxidation peak at 0.7V place, according to the relationship between the oxidation peak current and concentration, a method of electrochemical analysis called cyclic voltammetry was established to detect octyl phenol. The conditions of detection method for octyl phenol were optimized. The results showed that the optimal determination of the bottom fluid was the phosphate buffer solution (pH=5.0) and scanning rate was 100mV/s. In the range of 7.0×10-7~3.1×10-5mol/L, concentration and oxidation peak currents of ocytl phenol presented a good linear relationship, the linear equation was: ipa (A)= 2.0×10-6+0.4598c (mol/L), the linear correlation coefficient was r=0.9966 and this detection limit of this method was 4.0×10-7mol/L. Standard addition method was used to the detection of recovery of octyl phenol samples, the recovery range was 96.5% ~ 105.4%, and the average recovery was 99.7%. The method applied to detect the content of octyl phenol in carp body had taken good results. The advantages of this method were convenient, preparation for electrode was simple and determination result was accurate, etc.
Keywords: Carbon paste electrodes; Octyl phenol; Cyclic voltammetry；Carp