乙醇和異丙醇對程序降溫盒溫度變化的影響 

          

摘  要：傳統(tǒng)的梯度降溫冷凍方法對細胞的損傷嚴重，細胞復(fù)蘇后成活率低，活力差。程序降溫法降溫速度較緩，對細胞的損傷小，保存的長期性和安全性均可得到保障。細胞的體外長期保存一般采用10%二甲基亞砜低溫保護劑, 程序降溫后貯存在液氮中。但傳統(tǒng)的程序降溫法需要購置昂貴的程序冷凍儀，因此，程序降溫冷凍保存難以在多數(shù)實驗室中進行推廣。現(xiàn)制作一種簡易的程序降溫盒，以實現(xiàn)便捷經(jīng)濟地程序降溫。通過分析尋找出乙醇和異丙醇可作為程序降溫液，本實驗通過對比乙醇和異丙醇以及乙醇和異丙醇1：1的混合液的溫度變化速率，得出乙醇和異丙醇的混合液的降溫速率慢，最適宜作程序降溫液。

    關(guān)鍵詞：程序降溫盒； 乙醇； 異丙醇； 溫度變化

Effect of ethanol and isopropanol on programmed cooling box temperature

Abstract: Freezing method of ladder often use of today's cooling on cell damage, the low survival rate of cells after resuscitation, poor activity. Using the method of program cooling speed is slow, has small damage to the cells. The long-term preservation and safety can be guaranteed. The long-term preservation of cells in vitro by cryoprotective agent 10% two dimethyl sulfoxide, programmed cooling after storage in liquid nitrogen, but the traditional programmed cooling method requires the purchase of expensive program freezing equipment, therefore, the programmed cooling cryopreservation is difficult to spread in most laboratories. We are looking for a cooling speed slow solution, design program cooling box, to realize quick economic programmed cooling. Through the analysis to find out the more suitable for ethanol and isopropanol, through the contrast of ethanol and isopropyl alcohol and their 1:1 mixture temperature change rate, we get that the ethanol and isopropanol mixture significantly slower cooling rate.

Key words: programmed cooling box ；ethylalcohol；isopropanol；temperature shift

目    錄

1. 引言（緒論）..........................................................1

2.材料與方法...........................................................2

2.1 實驗材料..........................................................2

2.1.1 試劑...........................................................2

2.1.2 主要儀器和設(shè)備.................................................2

2.2 實驗方法..........................................................2

2.2.1制作程序冷凍盒..................................................2

2.2.1.1尋找合適的材料................................. .............2

2.2.1.2制作冷凍管的固定板...........................................2

2.2.2程序降溫液......................................................2

2.2.2.1 程序降溫液的篩選............................................2

2.2.2.2程序降溫液的處理.............................................2

2.2.3 測量溫度變化...................................................3

2.2.4 實驗結(jié)果觀察...................................................3

3.實驗結(jié)果.............................................................3

3.1 不同材質(zhì)的對比....................................................3

3.2 程序降溫液的篩選.................................................3

3.3 比較三種溶液的溫度變化...........................................3

4.結(jié)論（結(jié)束語）........................................................4

參考文獻..............................................................5

致謝 .................................................................6