摘    要

　　　豬鏈球菌病是由鏈球菌感染所引起的豬的一類疾病的總稱。豬鏈球菌急性常為出血型敗血癥和腦膜炎，慢性的以關(guān)節(jié)炎為特點(diǎn)。但是近年來鏈球菌病的非典型癥狀增多，病原菌的耐藥性逐漸增強(qiáng)。本試驗(yàn)選用六種常用抗菌藥物對(duì)從某豬場的病料中分離到的豬源鏈球菌進(jìn)行體外抗菌活性研究，結(jié)果表明分離菌株對(duì)氨芐西林、阿莫西林高度敏感，對(duì)紅霉素、慶大霉素中度敏感，對(duì)恩諾沙星、青霉素具有耐藥性。因此臨床用藥時(shí)應(yīng)根據(jù)藥敏試驗(yàn)結(jié)果選擇用藥，以獲得最佳治療效果。

　　　

　　　關(guān)鍵詞：抗菌藥；鏈球菌；體外抗菌活性

The experiment of 6 types of popular antibacterial drugs to make an in vitro chromatographic analysis on the swine streptococosis.

Name: Zhou Lei

                Major: Animal and Plant Quarantine

  Tutor: Yanqiu Luo

　　　　　　　　　　　　　　　　

Abstract

　　　Swine streptococosis is the general name of one swine disease caused by the streptococcal infection. Acute swine streptococosis is usually characterized as hemorrhagic septicemia and brain fever, and chronic as arthritis. But recent years, streptococcicosis has more and more atypical symptoms, and the drug resistance of pathogenicbacteria is growing strong. This experiment chooses 6 types of popular antibacterial drugs to make an in vitro chromatographic analysis on the swine streptococosis which is isolated from specimens in one hoggery, the result shows that strain isolation will be highly sensitve to ampicillin,amoxicillin; moderately sentive to erythrocin，gentamicin, and drug resistance to enrofloxacin penicillin. thus, it should be upon the drug sensitiveness test result to choose medicine in the clinical medication to achieve best treament effect.

Key Words：Antibacterial drugs；drug sensitive test；In vitro antibacterial activity

目    錄

摘    要 I

Abstract II

前言 1

1  試驗(yàn)材料 3

　　1.1病料……………………………………………2

　　1.2 培養(yǎng)基 3

　　1.3 藥敏片 3

　　1.4 抗菌藥物 3

　　1.5 無菌試管 3

　　1.6 試驗(yàn)動(dòng)物 3

　　1.7 試驗(yàn)儀器 4

2  試驗(yàn)方法 5

　　2.1 細(xì)菌的分離培養(yǎng)及鑒定 5

2.2 培養(yǎng)特性試驗(yàn) 6

2.3 生化試驗(yàn) 6

2.4 溶血試驗(yàn) 6

2.5 動(dòng)物致病性試驗(yàn)……………………………...…..3

2.6 藥敏試驗(yàn) 6

　　　　2.7 最小抑菌濃度的測定 7

3  結(jié)果與分析 9

　　3.1 細(xì)菌分離 9

　　3.2 培養(yǎng)特性 9

　　3.3 生化試驗(yàn) 9

　　3.4 溶血性試驗(yàn) 10

3.5 致病性試驗(yàn)………………………...………..6

3.6 藥敏性試驗(yàn)………………………………………….........6

　　　　3.7 最小抑菌濃度試驗(yàn)……………………………..7

4  討論…………………………………………...8

    結(jié)    論……………………………………………………….9

參考文獻(xiàn)…………………………………………………...10

致    謝……………………………………………………...11