千層塔中石杉?jí)A甲的分離、純化研究報(bào)告

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摘 要

本文研究了從千層塔中分離、純化石杉?jí)A甲的方法。發(fā)現(xiàn)采用大孔吸附樹脂法、液-液萃取及反萃取方法可較好的分離、純化石杉?jí)A甲。研究結(jié)果表明：將石杉?jí)A甲粗提液pH調(diào)至7.5后上柱，
采用pH為5.0、濃度50%的乙醇進(jìn)行洗脫，柱分離效果最好；柱分離產(chǎn)品的得率和純度分別為0.6113%和16.77%。然后將分離液在pH3.0及pH9.0時(shí)分別用氯仿進(jìn)行萃取，可得到石杉?jí)A甲含量較高的萃取物；萃取后產(chǎn)品的得率和純度分別為0.1495％和52.23％。

關(guān)鍵詞

千層塔,石杉?jí)A甲,分離,純化


Abstract

The method of isolation and purification of Huperzine A from the Huperzia serrata was studied in this paper. It was found that the macroporous absorption resins, the liquid-liquid extraction and the reverse extraction could isolate and purify Huperzine A effectively. The results showed: the best column isolation condition of Huperzine A is that the pH value of the roughly extracted from Huperzia serrata was adjusted to 7.5 and then eluted by 50% ethanol resolution at pH5.0; The yield rate and purity of the isolated product were 0.6113% and 16.77% respectively. Then the pH value of the isolated of Huperzia serrata was adjusted to 3.0 and 9.0 and extracted by chloroform respectively; the content of Hup A of the product was higher. The yield rate and purity of the purified product were 0.1495% and 52.23% respectively.

目錄：
前言
１．材料與方法
２．結(jié)果與分析
2.1 石杉?jí)A甲柱分離條件的研究
2.2 萃取條件的研究
3．結(jié)論
致 謝
部分參考文獻(xiàn)

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118-121
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